The Sociological Imagination, By C. Wright Mills - 1692 Words

In today’s culture, roughly 36% of Americans earn a college degree, while the remaining 64% are either presented with different options, or simply do not have the resources to go to college (Butler-Sweet, September 5, 2017). It is often assumed that a person’s college status, as well as their entire life, can be determined by examining his or her intellect, high school performance, and talents. However, C. Wright Mills proposes a new approach to this idea in his work, â€Å"The Promise.† Mills presents an idea known as the sociological imagination, which examines society on a larger scale to better grasp an individual’s life circumstances (Mills 2). The sociological imagination examines the role of social forces on the lives of individuals†¦show more content†¦As a female middle-class Caucasian, due to American societal oppressions such as male domination, racism, and socioeconomics, my chances at earning a college degree are high. Furthermore, due to the constant historical changes that take place, my status as a college student would not have been the same seventy- five years ago. By further examining my gender, class, and race using the sociological imagination, the significance behind my status as a member of the 36% of Americans granted the privilege of a college education is revealed. As a female, I am more likely to go to college than a male. Approximately 57% of college students are female, while only 43% of college students are male. Women are required to earn a college degree to earn a livable income. Men, however, are presented with more options than college alone. At graduation, males have the option of trade school or the military, as well as a college education. Jobs in the military and in the trades yield a livable income, while female job opportunities that require training rather than a degree (such as a dental hygienist or hair stylist) do not yield a livable income (Butler-Sweet, September 12, 2017). Although my chances at earning a college degree are high in the modern world, seventy-five years ago the option of going to college did not exist for women. Therefore, this option would not have existed for myself. The adult femaleShow MoreRelatedThe Sociological Imagination By C. Wright Mills857 Words   |  4 PagesThe sociological imagination is simply the act of having the capacity to think ourselves away from the commonplace schedules of our day by day lives keeping in mind the end goal to take a gander at them with a new perspective. C. Wright Mills, who made the idea and composed a book about it, characterized the sociological creative ability as the clear attention to the connection amongst encounter and the more extensive society. The sociological imagination is the capacity to see things sociallyRead MoreSociological Imagination By C. Wright Mills969 Words   |  4 Pages C. Wright Mills defined sociological imagination as the awareness of the relationship between per sonal experience and the wider society. Understanding and being able to exercise the sociological imagination helps us understand the relationship between the individual and society. Mills focuses on the distinction between personal troubles and public issues. Having sociological imagination is critical for individual people and societies at large to understand. It is important that people areRead MoreThe Sociological Imagination : C. Wright Mills907 Words   |  4 Pagesindividual s life a person will experience what C. Wright Mills refers to as the trap. The trap alludes to a person that can only see and understand their own small scope of life. Their frame of reference is limited to their day to day life and personal experiences that are directly related to them, they cannot see the bigger picture. They do not yet know that the sociological imagination can set them free from this trap and as C. Wright Mills said, In many ways it is a terrible lesson; in manyRead MoreThe Sociolog ical Imagination By C. Wright Mills1315 Words   |  6 Pagesâ€Å"The sociological imagination enables us to grasp history and biography and the relations between the two within society. This is its task and its promise.† C. Wright Mills writes about the sociological imagination in an attempt to have society become aware of the relationship between one’s personal experience in comparison to the wider society. By employing the sociological imagination into the real world, individuals are forced to perceive, from a neutral position, social structures that, inRead MoreThe Sociological Imagination By C. Wright Mills986 Words   |  4 PagesMills Chapter Summary â€Å"Yet Men do not usually define the troubles they endure in terms of historical change and institution contradiction.† Stated from chapter one of â€Å"The Classic Readings in Sociology† which was based on â€Å"The Sociology Imagination† by C. Wright Mills. As our Sociology 131 class study the works of C. Wright Mills, we learn and examine his views. We learn how he view other things such as marriage, war, and the limitations of men. His view of war is that both sides playRead MoreSociological Imagination By C. Wright Mills942 Words   |  4 PagesSociological imagination according to C. Wright Mills (1959) â€Å"enables its possessor to understand the larger historical scene in terms of its meaning for the inner life and the external career of a variety of individuals† (p.5) Mills in this book of The Sociological Imagination explains how society shapes the people. Mills wants people to be able to use sociological imagination to see things in a sociology point of view, so they can know the difference between personal troubles versus personal issuesRead MoreSociological Imagination, By C. Wright Mills Essay1611 Words   |  7 PagesI SOCIOLOGICAL IMAGINATION CONCEPTUALIZATION As conceived by C. Wright Mills, sociological imagination is the mental ability to establish intelligible relations among social structure and personal biography that is observing and seeing the impact of society over our private lives. Sociological imagination helps an individual to understand on a much larger scale the meaning and effect of society on of one’s daily life experience. People blame themselves for their own personal problems and they themselvesRead MoreThe Sociological Imagination, By C. Wright Mills799 Words   |  4 Pages The sociological imagination, a concept used by C. Wright Mills, is essentially the ability to perceive a situation or act in a much larger social context as well as examining the situation or act from many perspectives. In particular, it plays a paramount role in Donna Gaines Teenage Wasteland. It is a tragic story of 4 teens who together, committed suicide. The teens were deemed as â€Å"dropouts, druggies† [Teenage Wasteland 8.2 ] by newspapers and were still treated with disdain even after theirRead MoreThe Sociological Imagination : C. Wright Mills1822 Words   |  8 PagesC. Wright Mills defines the sociological imagination as, â€Å"what they need, and what they feel they need, is a quality of mind that will help them to use information and to develop reason in order to achieve lucid summations of what is going on in the world and of what may be happening within themselves†. Mills also says that the sociological imagination enables us to grasp history and biography and the relations between the two within society. When I read Chapter One: The Promise from C. WrightRead MoreSociological Imagination, By C. Wright Mills1762 Words   |  8 Pages 10/11/2017 ID 100602667 Soc. 1 FY40 Sociological Imagination The Sociological Imagination, by C. Wright Mills, was a statement that questioned the developing field of sociology, challenging sociologists and the public to take seriously the rise of elites and the decline of American democracy, American community, and American equality. Mills argues that the sociological imagination is a quality of mind necessary to the understanding of the human condition

Renaissance And The Italian Renaissance - 1396 Words

The Italian Renaissance had two distinct periods during its lifetime. The first being the â€Å"Early Renaissance†, which took place between the late 13th and the early 14th centuries. The second period was known as the â€Å"High Renaissance†, which took place during the 15th and 16th centuries. These two periods had very distinct foundations and styles behind the art and architecture of their respective periods. The Early Renaissance revived many old themes which later became the foundations for the High Renaissance. While the High Renaissance strayed from previous traditions and had its own form of art and essentially perfected early themes and styles of art and included more scientific/mathematic components. The Early Renaissance saw the†¦show more content†¦Architects and designers such as Brunelleschi, Alberti, and Rossellino were absolutely obsessed with designing and engineering building with perfect symmetry, proportions, and with ample amounts of geometry. These architectural styles can be seen in the Pazzi Chapel (to the left), which was designed by the famous Filippo Brunelleschi. The Palazzo Rucellai (to the right) (designed by Alberti and architected by Rossellino) in Florence, Italy is another example of architectural uniformity during the Early Renaissance. Proportions can be clearly seen throughout the exterior of the building and reflects Roman architecture, which Alberti admired heavily. The most complex and most high-profile masterpieces and artists to come out of the Italian Renaissance came during the 15th and 16th centuries, which was also known as the High Renaissance. The architectural and artistic styles of the Early Renaissance essentially laid the foundations for the High Renaissance period of art and architecture. These styles were mostly perfected during the High Renaissance. One such example of perfected art is the most famous portrait known as the Mona Lisa by Leonardo da Vinci. The Mona Lisa â€Å"brings figure and landscape into exquisite harmony: the pyramidal shape of the sitter is echoed in the curves of distant roads and rivers.† (â€Å"Renaissance/Baroque Humanities† 59). TheShow MoreRelatedThe Italian Renaissance And The Renaissance1424 Words   |  6 PagesThe Italian Renaissance was an explosion of art, writing, and thought, that roughly lasted between 1300 to 1600. In this time each citizen, countrymen, or villager had and per formed different jobs and careers. Humanism the study of Greek and Roman writings, art, and architecture, initially jump started the Renaissance, and the need for art. Artists now were inspired to use life like art and linear perspective, so art seemed and was more realistic. The start of the Italian Renaissance was theRead MoreItalian Renaissance And The Renaissance910 Words   |  4 PagesIn the late 14th century to the 16th century, the Italian Renaissance was taking place and moving from the Middle Ages at a time when the Catholic Church was indomitable to a period called The Renaissance. After this â€Å"rebirth† in Italy began, it started spreading to other countries to then produce a French Renaissance, an English Renaissance, and so on. Italy served a sort of birth place for the Renaissance in European culture. During this time period, more and more people were placing humans asRead MoreThe Renaissance And Italian Renaissance961 Words   |  4 Pages The Renaissance (rebirth), was a time in which all art became more personalized and represented more than we see. There were two major stages in the Western European/ Italian Renaissance: Early Renaissance and High Renaissance. It gave patrons a new outlook on art and the world. Styles such as Mannerism, Fresco, and Parietal help advance art in western Europe. Most of the Renaissance works were religious,or had had something to do with the ancient times (ancient greece and roman culture). Not onlyRead MoreThe Italian Renaissance And The Renaissance1158 Words   |  5 PagesThe Renaissance period is known for the revival of the classical art and intellect born in ancient Greece and Rome. The Renaissance is also a time that is marked by growth, exploration, and rebirth. The Italian Renaissance started in Florence and progressively made its way into Venice and then into the great city of Rome. During the Renaissance, Rome was home to some of the renowned works of art and the finest ar chitectural masterpieces in the world - too many that still holds true today. Along withRead MoreThe Italian Renaissance1558 Words   |  7 Pages Julianna Plunkett Ms. McGuire British Literature 11 06/10/15 The Italian Renaissance began in the mid-fourteenth century, and was marked by a turn from medieval life and values dominated by the Church toward the philosophical principles of humanism. The Italian people, especially the educated middle class, became interested in individual achievements and emphasized life in this world, as opposed to preparation for life in the next world, which was stressedRead MoreThe Italian Renaissance870 Words   |  4 Pagescentury, Italy was divided into many self-governing city sates. Florence, an independent republic and third largest city in Europe at the time, was the motherland of the Italian Renaissance. This was an enlightening time of â€Å"rebirth† that began directly after the Middle Ages (History.com Staff). Florence was the birthplace of the Renaissance due to its location, prosperity, and cultural amalgamation. WHY IS ITS LOCATION SO SPECIAL - why not somewhere else? Italy consisted of communes, or city-states, ruledRead MoreThe Italian Renaissance1228 Words   |  5 PagesThe Italian Renaissance was a time for great cultural change and achievements, which began in Italy approximately during the 13th century and lasted up until the 16th century. It marked the transition between the Dark Ages and Early Modern Europe. The European Renaissance originated in Central Italy, and centered in the city of Florence.i The distinctive characteristics of northern Italian states such as art, literature, philosophy, and culture produced an atmosphere of learning and artistic expressionRead MoreComparing The Italian And Italian Renaissance1748 Words   |  7 PagesNorthern Renaissance and Italian Renaissance Differences between the Italian and Northern Renaissance The Renaissance was a period of great cultural and technological changes which swept Europe from the end of the 13 century. It was integral in developing Europe into a powerhouse. Although, each part of Europe was subjected to different changes, there were two primary renaissances which were most notable. They were the Italian and the Northern renaissance. Both of these renaissances had a profoundRead MoreChildbirth And The Italian Renaissance1741 Words   |  7 PagesChildbirth and Childbearing in Renaissance Italy for upper status woman, was far different than what childbearing is compared to today’s standards. In renaissance Italy it was said that woman only had one purpose and that purpose was to have many legitimate babies, especially for the nobility and upper status couples. From having these babies, the family could inherit a lot of wealth (King, M. L., 2003). This differs greatly in todays society, as now woman’s primary function is not only to produceRead Mor eThe Rise Of The Italian Renaissance1287 Words   |  6 PagesThe Italian Renaissance, in contrast to the conservative attitude of the Middle Ages, introduced a society dominated by a secular, humanistic spirit. One of the most historically significant events of the Renaissance was the Reformation. Some people argue that the Reformation was a rejection the secular spirit of the Italian Renaissance. Others assert that the Reformation was an embracement of these secular ideas. However as with many questions, this issue cannot be given a definite answer. The events

My mom is a wonderful woman Free Essays

Have you ever had that one person that you have looked up to your whole life? Well i have, and that person is my mom. My mom is a wonderful woman. She puts up with my attitude and all of the annoying things i may put her through, and she is still there for me when i need her. We will write a custom essay sample on My mom is a wonderful woman or any similar topic only for you Order Now My mom is someone that i love and that i can rely on if i need something. I know she will never let me down and that is why i can depend on her for anything. I can trust my mom and that is a problem i have with other people. I have never really been able to trust anybody. My mom has helped me a lot in life whether its figuring out how to do something, helping me achieve my goals, or telling me not to give up. I can ask my mom for advice and talk to her about anything. My mom is the main reason that i am doing sports in high school. I have always wanted to do sports but i have always been shy and scared to try new things. I didn’t want to try out because i was afraid i wouldn’t make the team. She encouraged me to try and and i made the team thanks to her. I can talk to my mom about anything. My mom was there for me when i switched schools and i didn’t know anybody. I would talk to her and she would tell me just be your self and don’t let anybody change you. She is someone i constantly go to when i have no one to talk to. We might play around a lot but I know when she is joking and i know when she is being serious with me. My mom is the best person in the world. My mom has always believed in me. She told me to always try my best. She told me that even if i feel like I’m going to fail to always try me hardest because i never know what could happen. She also told me to never give up. She told me that before i started doing sports that as long as i try my hardest she will supply me with everything i need to succeed. My mom has helped me make a lot of decisions. She helped me figure out that i wanted to join a sport, she helped me decide if i wanted to do an honors class or not, and she talked to me about joining and advanced placement class. My mom is the main reason i am the person i have grown up to be. If she wasn’t her for me i would have probably been a totally different person. Even though i get on my moms nerves all the time she still puts up with me and she still loves me. There is a couple of times were we have argued with each other, but we easily get over that. I look up to my mom and i hope that when I’m a mom my kids will look up to me like i look up to my mom. How to cite My mom is a wonderful woman, Essay examples

Corporate Social Responsibility Complementary Frameworks

Question: Discuss about the Corporate Social Responsibility for Complementary Frameworks. Answer: Introduction Corporate social responsibility and ethics are important for an organization to survive. Globalization has led to multiple opportunities but it has given importance to development of ethical conduct and good governance. CSL group Limited is a global biotechnology company which aims for saving life by research and development whereas Macquarie is an investment company which aims for creating wealth for its investors. Both the company has different operations but there way to achieve success is by following value and ethics. CSL CSL is a global biotechnology company founded in 1916 in Australia. Commonwealth serum laboratories is a government held company dedicated in research, development, and manufacturing of medical products. They are known for producing vaccines, antivenom and blood plasma derivatives through medical investigation. It has employed 16,000 employees in more than 30 countries and has medical facilities in Australia, Germany, Switzerland, U.K, and U.S.A (CSL Group Global, 2016). Macquarie group Macquarie group is the largest Australian global investment bank which is providing multiple banking, financial advisory, merger and acquisition, and fund management services to the world. Macquarie has its headquarters in Sydney and is listed on Australian Securities Exchange. It is known as the Millionaire Factory because of its lucrative rewards and high margin profits (Our organization, 2016). Mission Vision CSL mission is to ensure safety, quality to its customers through extensive research and development. There focus is to protect the health of the people and save them against hazardous ailment. It has grown into a leader providing pioneering therapies to the patients around the world. The company aims for creating a difference by saving the lives with the help of its dedicated staff (About CSL, 2016).CSL is consistently focused on achieving their goals by keeping them simple and measurable. There specific goal is to ensure safety of the patients which they have achieved with their realistic approach under the given time period. They are popular for providing niche services in a biotechnology industry. Their global presence has helped them to achieve the prerequisite mission. They are consistently expanding their business which is held for their success. Macquarie vision is to enhance the wealth of its investors by diversifying the assets at various places. They are focused on uncovering the opportunities through consistent encouragement. Innovation and entrepreneurial drive is their mission to achieve success under competitive environment. Macquarie is focused on enhancing the wealth of the shareholders and investors through risk management tools. Their goals are big which sometimes poses difficulty to achieve a regular growth. A report has shown that they are addressed by media as the Millionaire Factory due to their timely dedication in following the realistic approach. But it is helping them to achieve regular growth. Values ethics CSL is focused on meeting the needs of its customers through consistent inventions in the field of biotechnology. The group is driven by ethic and honest to pursue its mission regarding health care and research. For the purpose of providing better health facilities the company has collaborated with other biotech giants to save life through research. Their superior quality performance in the field of biotechnology has forced them to focus on maintaining value and ethics. The company is focused on emphasizing on the ethics and morals which is the inherent mission of the company. Ethics and morals are given priority with the change in global environment (Value and Ethics, 2016). CSL has a clear values and ethics which they have achieved within the given time frame. It is due to their consistent dedication they have achieved a remarkable growth. The company is working for the welfare of the society by bringing changes in the medicine industry whereas; Macquarie aims for enhancing the ove rall wealth of their investors. They are focused on achieving the required growth as per the trends. The mode of operations and values of both the company are different while measure on same parameters. Macquarie works in the favor of its investor on the principles of honesty and transparency. Their high quality standards have helped them in earning the trust of the clients, community, and shareholders. They value to encourage high ethical atmosphere in managing the wealth of its clients. All these features have helped them in achieving the desired goals through regular improvement in the services. The company has a strict framework against any unethical activities. Its financial division has developed internal values by developing collaboration and transparency. They are consistently focused on building strong relationship with the clients. The staff is given rights to harness relationships with the clients. Corporate Responsibility CSL is focused on conducting its business through ethical practices by making regular contribution in the social, economic and environmental well-being. Sustainable environment is their priority while delivering its services. They ensure that their CR practices are inculcated in their regular functioning to benefit the interest of their stakeholders. There key areas are to focus on the research and development by meeting the needs of the patients. CSL ensures to manage high standard of product life cycle by incorporating best CSR practices in their work- culture. It includes that their medicines and other products are reaching its customers in an ethical manner by dispersing the financial success with their shareholders. The management has ensured that the research and technology is not imposing any kind of harm to the environment. CSL is committed to comply with the regulations in meeting its research code of conduct. They ensure that their research does not harm the rights of the p articipants (Corporate responsibility, 2016). In addition proper monitoring is done when experiments are conducted on the animals. This provides an insight into the corporate social responsibility and governance practices. Macquarie structure is based on ESG approach which gives special attention towards managing environmental and social risk. The employees are focused on delivering best services to the clients through strict business practices. Their environmental social risk is based on the international guidelines to provide the customers with the world-class environment. They have incorporated high standards in their business conduct by focusing on its three pillars (opportunity, accountability, and integrity). There business practices are carried with high degree of consistency with the law and market regulations which has helped in committing their assets into correct place. They are focusing their investments into the sustainable development of the environment with the support of their clients. A major amount is invested in the field of technology, infrastructure and renewable energy. The corporate social responsibility of CSL is based on the simplistic approach. As per the report they are follo wing the strict norms as mentioned in their website. The company is adhering to the norms as mentioned by the government whereas; Macquarie approach is based on the social responsibility mentioned in their memorandum. Both of the companys way of achieving the value is different but both of them are making a difference to the society by making investments in various projects. Engagement of stakeholders interest is the foundation of the corporate responsibility. At CSL the key stakeholders are patients, employees, investors, regulators, government healthcare professional, plasma donor, academic staff and partners. It includes different educating the patients, health care services, active collaboration to form a structured organization. They believe in following different mechanism as per the current issues which are involved in the well-being of the organization (Caroll, A.2015) (MasonSimmons, 2014). Macquaire mission is to focus on enhancing the wealth of its shareholders by incorporating the best governance practice. Their roadmap is to enhance the wealty of the shareholders by diversifying it at different places. clents, shareholders, community is their utmost priority while managing wealth. The various stakeholders are government, regulatory frame work, consumers, clients, investors, shareholders, etc( Leadership and corporate governance.2016).They are persistent towards delivering the best services by adhering on the responsibility which are on them while developing the wealth of the various stakeholders. The stakeholders of both the organization are different in terms of their opertaional areas. CSL is a bioseutical company held by the australian government. The major stakeholders of this company are mostly research department, patients, research partners, licence partners etc whereas in case og macquaire the investor criteria is completely different while interpreting the result. on the parameter of SMART Aanalysis both the company are fulfilling the stakeholder criteria (Business Studies.2014). Both the organization has a different set of stakeholders but they are trying their best to satisfy the needs of their customers. It is hence proved that both the companies are making consistent effort by benifitting the stakeholders (Schaefer and Williams, 2013) Comparison Similarity Both are working in a complete different environment but are committed in achieving the requisite growth. There ways of achieving the goals can be seen through their corporate statement. Where CSLis focused on saving the life of the people through innovationn and research, macquaire has a record to benefit the stakeholders through continuous hardwork. There employers are creating continuous difference to the society which helped both the companies to achieve a remarkable growth in the recent time. Conclusion The intentition of both the company is mentioned in its charter which focues on achieving result with a mutual corporation among the organization with its stakeholders. Both the organization has a different goals and objectives but they are focused on achieving a sustainable environment through its consistent hard work. Their intention are believable and ensures achievement of multiple target with keeping in mind the corporate social responsibility which is mentioned in their corporate report. The company has dedicated itself for the well being of the environment which is necessary to be fulfiled by adhering to the goals. Both the companies have a set of objectives which can only be incorporated if focused with the desired result. References CSL Group Global, 2016. (Online). Available from: https://www.csl.com.au/ (Accessed on: 28/09/16) About CSL, 2016. (Online). Available from: https://www.csl.com.au/about (Accessed on: 28/09/16) Value and Ethics.2016. (Online). Available from: https://www.csl.com.au/about/values-ethics.htm (Accessed on 28/09/16) Corporate responsibility, 2016. (Online).Available from: https://www.csl.com.au/corporate-responsibility.htm (Accessed on: 28/09/16) Caroll, A.2015. Corporate social responsibility: The centerpiece of competing and complementary Frameworks. Organizational Dynamics (2015) 44, 87-96 Schaefer, A. and Williams, S. 2013.Small and Medium-Sized Enterprises and Sustainability: Managers' Values and Engagement with Environmental and Climate Change Issues. Volume 22, Issue March 2013, Pages 173186 Mason, C. Simmons, 2014. Embedding Corporate Social Responsibility in Corporate Governance: A Stakeholder Systems Approach.2013.Journal of Business Ethics (2014) 119: 77. Colley, S.2013.An examination of the relationship amongst profiles of perceived organizational values, safety climate and safety outcomes. Safety Science Volume 51, Issue 1, January 2013, Pages 6976 About us, 2016. (Online).Available from: https://www.macquarie.com/au/about (Accessed on: 28/09/16) Our organization, 2016. (Online).Available from: https://www.macquarie.com/au/about/company#our-organisation (Accessed on: 28/09/16) Leadership and corporate governance.2016. (Online).Available from: https://www.macquarie.com/au/about/company/leadership-corporate-governance (Accessed on: 28/09/16 Business Studies.2014. (Online).Available from: https://www.bbc.co.uk/schools/gcsebitesize/business/aims/partnershiprev2.shtml (Accessed on: 28/09/16)

Early Civilizations Flashcard

Early Civilizations

Maths Cars coursework Plan Essays

Maths Cars coursework Plan Essays Maths Cars coursework Plan Essay Maths Cars coursework Plan Essay The data base contains information about some used cars. I will be using the information to investigate what influences and affects the price of second hand cars. I will be investigating the price, age, and mileage of the cars. Also I will choose three types of cars and compare their prices. The types of cars are: small cars, executive cars and sports cars. I will need to collect data on these cars and then I am going to compare their ages using comparative pie charts. To do this I will need to make sure the pie charts areas have the same ratio as their totals. For that I have to choose the radius for the smallest pie chart and work out the radii for the other pie charts based on the previous ratios and my chosen radius. I will also need to see the correlations between Age and Price, and the correlations between, Mileage and Price. This will be done to see if they have a negative correlation or positive correlation. This will be done using scatter graphs and h ow strong each factor influences prices by spearman coefficient of rank correlation. My hypotheses for this investigation are: 1. There is a negative correlation between age and price. 2. There is a negative correlation between mileage and price. 3. Executive cars are in general more expensive then sports cars. 4. Sports cars tend to be more expensive then small cars. 5. The correlation between age and price will be stronger for small cars. 6. The influence of price on age will be stronger then influence of mileage on age. I will need to select samples from all the cars and from the types of cars I have chosen to investigate further. I will select stratified samples from all the cars and random from the types of cars I have chosen. I will use random samples for part C, D, E, F, G and from each group I have chosen to investigate and stratified samples for parts A and B. to do this I will use the computer to randomise the data to get random samples, however the samples may produce some outliers (very old cars with very high prices, Rolls Royce or Jaguar etc.) such data I will ignore but I will make a note of the numbers of outliers. I will plot each sample on scatter graphs (one factor against price) 8 scatter graphs will be produced: 1. stratified sample: age, price 2. small cars: age, price 3. executive cars: age, price 4. sports cars: age, price 5. stratified sample: mileage, price 6. small cars: mileage, price 7. executive cars: mileage, price 8. sports cars: mileage, price I will draw lines of best fit on each scatter graph, working out mean first. I will work out spearmans coefficient of rank correlation for each scatter graph. I will use y = mx + c to work out equations of line of best fit. I will use the lines of best fit to predict prices given age or mileage for each type of car that I have chosen. I will intercept the meaning of gradient and use it to compare depreciations. I will work out mean price and range for each type of car and set out in a table together for easy comparison. Nxst I will use cumulative frequency graphs to compare prices of small, executive and sports cars. My recorded prices in grouped frequency table to compare prices of my chosen cars will be set out in this table: Sports Small Large Prices Tally Frequency Tally Frequency Tally Frequency à ¯Ã‚ ¿Ã‚ ½0-499 à ¯Ã‚ ¿Ã‚ ½500-1499 etc. And cumulative frequency table: Up to Sports cars cumulative frequency Small cars cumulative frequency Medium cars cumulative frequency à ¯Ã‚ ¿Ã‚ ½499 à ¯Ã‚ ¿Ã‚ ½1,499 Etc. Next I will plot cumulative frequency graphs on one graph paper (using different colours) and find quartiles Q1, Q2, Q3 for each type of car. I will then draw box and whisker diagrams using different colours against one scale. Then I will make comparisons of prices using medians and by using interquartile ranges. Then finally I will compare the distribution of prices of small, executive and sports cars using histograms with unequal intervals.

Thermal Storage Systems Research Paper Example | Topics and Well Written Essays - 500 words

Thermal Storage Systems - Research Paper Example A basic thermal storage system â€Å"cools a storage medium then saves the stored cool medium to be reused at another time.† (Washington State University) What is meant by defining thermal storage systems is simply that energy is stored and then used at a later time. The storing of this energy allows the user to save. The energy that was saved is not wasted and can be used when needed. Many traditional heating and cooling systems waste too much energy. That is why thermal storage systems are so convenient. What would normally be wasted is saved and used for later. The main way that a thermal storage system can be used is by allowing energy to be stored and saved for later. A residential installation of thermal energy storage systems can help cool a house. A home can store energy in the thermal energy storage system at night when it is cool, and then distribute the stored energy during the day when it is most needed. This is a money saving way to cool a home. This same process will also apply to small commercial businesses. The system can be modified to fit existing systems but the system will require an area to be used to store energy. This storage area can be located in a crawl space, basement or a large enough utility room. Using thermal energy storage systems for air conditioning is complex in a way but easy to operate.

Unemployment in UK Assignment Example | Topics and Well Written Essays - 2250 words

Unemployment in UK - Assignment Example The history of unemployment in the UK tells that during Great Depression the rate of unemployment was very high in the country. It reached to the level of 25 percent but after that time, unemployment rate used to remain at a low level and with the help of different policies and strategies the government of the UK succeeded to attain more or less full employment level until the 1970s. However, after an oil prices fluctuation in the 1970s the unemployment rate was also badly affected and gradually it began to rise. The recent rise in the unemployment rate is highest during the last decade and the country is facing the worst situation with respect to unemployment crisis in the country (Pettinger, p2, 2009). The following table shows the United Kingdom unemployment rate during last three years and it is clearly seen in the table that unemployment rate is continuously increasing in the country with each passing year. The recent statistics also show that the unemployment is recorded at the highest rate in last eight years. The rate of unemployment is rising at an alarming pace due to which the year 2009 is also expected as a stagnant year for the economy of United Kingdom. (Monaghan, p1, 2008) The country while responding towards the global financial crisis is witnessing worst situation of unemployment and there is a high level of concerns and worries at all level regarding the rise of the unemployment rate. The following chart shows the current situation of unemployment in the country that is expected to rise even more. Unemployment is closely related with the economic development of a country. When an economy faces recessions, the rate of unemployment also increases. Thus, the economic recession is the major cause behind the high rate of unemployment in the UK and it is expected that if the UK will face deep economic recession in 2009 then the unemployment will also reach the level of 3 million.  

English and Spanglish Personal Statement Example | Topics and Well Written Essays - 750 words

English and Spanglish - Personal Statement Example Both Spanish and English occupy an important place in my life helping me to communicate and understand other people. In my life, language diversity begins with effective quality education, and whenever we will seek to create multifaceted cultural strategies in schools, they will be readily reflected in my social environments, for these are also shaped by those who graduate from schools and who are actively involved into various types of cultural initiatives that are closely aligned with the major goals in education. Effective communication is not possible without learning and demonstrating good communication skills. My impression is that the general attitude to this language group is hostility that is showed from the side of the white population. Amy Tan writes: "people in department stores, at banks, and at restaurants did not take her seriously, did not give her good service, pretended not to understand her, or even acted as if they did not hear her" (Amy Tan). The combination of t hese skills is very important for facilitating effective communication. As for good communication practices, these practices are important for ensuring that my needs and interests are being heard and addressed. What is fundamental for effective communication is "active" listening. Traditionally, good listening entails listening to the content of the dialogue. In the major part of the cases the context of the dialogue is comprised of the tone and physical gestures. These are used by people to express the interests of the group. My English differs from other Englishes so I use many Spanish words and phrases which help me to express my emotions and feelings. Usually, I use these phrases at home and with my friends who understand them correctly. So, I try to avoid Spanishisms while communicate with other people. At present, I belong to Spanish language community. The major specific feature of this community is that the people show a specific kind of attitude to the Spanish spoken population of the island. Similar to Amy Tan, I experience that "there are other Asian-American students whose English spoken in the home might also be described as "broken" or "limited." And perhaps they also have teachers who are steering them away from writing and into math and science, which is what happened to me" (Amy Tan). This fact is very important since it can push language bearers to survive under the effect of many factors. The survival of the Spanish language is the thing that closely depends on the activities of the whol e group. The person that took an active role in the activities of the organization was projected to make a number of rather negative evaluations. Even now the group of rather negative evaluations still continues to be placed on Patois. Language use of these nations continues to be one of the things that still matters. Traditionally, this thing requires people to make little effort to provoke comments. My own experience in working with Spanish group indicates that the remnants of this accent still exist in our society. The main reason for that is the uniqueness of the Spanish language. Even now the language continues to be spoken in the U.S. This tendency remains to be supported by many U.S. residents. The main reason why U still use Spanish often can be simply described as "enjoyment". Once, I was also in the situation close to making a significant linguistic misstep. The situation

Studies of Adoptively Transferred CMV-Specific T Cells

Studies of Adoptively Transferred CMV-Specific T Cells Group Method of Expansion/Selection Riddell, 1992, 1995 Expansion using CMV-infected fibroblasts Einsele, 2002 Expansion with CMV lysate Cobbold, 2005 Tetramer Selection using magnetic beads Micklethwaite, 2008 Antigen-presenting cells (Dendritic cells) transduced with an adenoviral vector encoding CMVpp65 Peggs, 2011 Selection of T cells secreting IFN-ÃŽÂ ³ after exposure to CMV antigen Blyth, 2013 Antigen-presenting cells (Dendritic cells) transduced with an adenoviral vector encoding CMVpp65 or Dendritic cells pulsed with HLA-A02-restricted peptide NLVPMVATV Quoted from (Hanley and Bollard, 2014). The ability to generate CMV, EBV, and adenovirus-specific CTL from the 20% fraction of a cord blood unit by using dendritic cells transduced with an Ad5/f35-CMV-pp65 vector as well as the cytokines IL-7, IL-12, and IL-15 was reported by Hanley and colleagues in 2009. Responding T cells were shown to be derived from the naÃÆ'Â ¯ve T cell population and responded to typical and atypical, novel CMV-pp65 epitopes. Later on, the ability to generate CMV-specific T cells from CMV-seronegative donors was reported by Jedema et al., 2011 and Hanley et al., 2013. Vaccination: On the basis of the cost to the health care system and the impact of the virus on human suffering, the development of an effective prophylactic vaccine to prevent CMV symptomatic congenital disease and/or to prevent disease in immunocompromised individuals is a high priority and would be a highly cost-effective measure (Khanna and Diamond, 2006). A successful vaccine strategy should aim to stimulate the innate and adaptive immune responses at the appropriate time. Both humoral and cell-mediated immune responses might be necessary to prevent congenital disease, whereas cellular immune response alone might be sufficient to prevent virus-associated complications in transplant patients (Khanna and Diamond, 2006). Cytomegalovirus exhibits a high level of molecular diversity and carries many immune evasion genes (Hansen et al., 2010). Thus, infection within a host can occur with multiple virus strains concomitantly, including at the time of initial infection, or sequentially (Renzette et al., 2011). Broad and cross-neutralizing cellular and humoral responses have therefore become a major goal of vaccine design (Arvin et al., 2004). Various strategies have been developed, though a vaccine against CMV remains elusive. CMV vaccines have been obtained using attenuated or chimeric viruses, DBs, recombinant proteins, DNA, peptides and/or viral vectors (poxvirus/adenovirus) (Khanna and Diamond, 2006). A number of subunit CMV vaccines tested in clinical trials targeted the abundant pp65 protein (Sylwester et al., 2005), which is expressed by CMV-infected cells both early and late after infection (La Rosa et al., 2012). Cytomegalovirus vaccines in clinical trials include: glycoprotein B subunit vaccines; alphavirus replicon particle vaccines; DNA vaccines; and live-attenuated vaccines. A variety of vaccine strategies are also being examined in preclinical systems and animal models of infection. These include: recombinant vesicular stomatitis virus vaccines; recombinant modified vaccinia virus Ankara; replication-deficient adenovirus-vectored vaccines; and recombinant live-attenuated virus vaccines generated by mutagenesis of cloned rodent CMV genomes maintained as bacterial artificial chromosomes in Escherichia coli (Sung and Schleiss, 2010). Trial of a subunit vaccine consisting of recombinant HCMV envelope gB with MF59 adjuvant: All HCMV-infected individuals have a significant proportion of neutralizing antibodies to HCMV being specific for epitopes on gB (Sung and Schleiss, 2010). A study of the use of HCMV gB vaccine plus MF59 adjuvant was reported. It was administered following a 0-, 1- and 6-month schedule (Pass et al., 2009). Although the study demonstrated that the gB vaccine could significantly reduce the risk of acquiring primary maternal HCMV infection, the study did not address the question of whether vaccine-induced HCMV immunity was equivalent to natural immunity in modulating either infection rate or sequelae for the fetus (Dekker and Arvin, 2009). Since re-infection with new strains of HCMV with which the host has no prior experience can lead to transmission to the fetus with subsequent sequelae (Boppana et al., 2001), the issue of cross-protection against diverse clinical isolates following administration of gB vaccine from a single genotype must be defined in future studies (Sung and Schleiss, 2010). Clinical trial evaluation of a two-component alphavirus replicon particle vaccine containing HCMV gB and phosphoprotein 65 (pp65)/immediate early fusion proteins: The gB and the pp65 are the most frequently recognized antigens by CD4+ T cells, and pp65 is also one of the antigens most frequently recognized by CD8+ T cells (Sylwester et al., 2005). The HCMV IE1 is also an important target of the CD8+ T-cell response (Slezak et al., 2007). Therefore, vaccination strategies that aimed at eliciting T-cell responses has focused on the pp65 protein andIE1 gene product (Sung and Schleiss, 2010). AVX601 is a two-component alphavirus replicon particle vaccine expressing HCMV gB and a fusion protein of pp65-IE1 (Reap et al., 2007). The vaccine was well tolerated, with only mild local reactogenicity, Mild-to-moderate systemic reactogenicity was reported in some subjects (Sung and Schleiss, 2010). Bivalent HCMV DNA vaccine: The use of a HCMV DNA vaccine in immunocompromised subjects, such as transplant recipients, would eliminate the safety concerns of live-attenuated HCMV or live recombinant viral-vectored vaccines (Selinsky et al., 2006). DNA vaccines elicit robust CD4+ and CD8+ T-cell and antibody responses (Sung and Schleiss, 2010). VCL-CB01, a bivalent HCMV DNA vaccine that contains two plasmids encoding HCMV pp65 and gB (LiuÂÂ   and Ulmer, 2005). This vaccine has the ability to prime antigen-specific T cells, with the capacity to proliferate and secrete IFN-ÃŽÂ ³ on restimulation with antigen (Wloch et al., 2008). Further modifications of this vaccine may be required to optimize immunogenicity, particularly to the gB moiety (Sung and Schleiss, 2010). It was generally well tolerated. The most common adverse event was mild site injection pain (Liu and Ulmer, 2005). Live-attenuated HCMV Towne vaccine with or without adjuvant recombinant IL-12 and/or priming by DNA vaccine: Immunization with Towne vaccine prevented HCMV disease in seronegative renal transplant recipients, although it did not prevent infection in these patients or in parents of HCMV-infected children (Sung and Schleiss, 2010). Evidence suggests that the relative defect in Towne vaccine may be related to inadequate antigen-specific IFN-ÃŽÂ ³ responses by CD4+ and CD8+ T cells following vaccination (Jacobson et al., 2006). Approaches to improve the immunogenicity of the Towne vaccine are being explored (Jacobson et al., 2009). One approach was to generate genetic recombinant vaccines containing regions from the genome of the unattenuated Toledo strain of HCMV, substituted for the corresponding regions of the Towne genome (Heineman et al., 2006). In another approach, HCMV DNA vaccine is used to prime for memory immune responses to Towne vaccine (Jacobson et al., 2009). A third approach is to co-administer Towne with recombinant human IL-12 (Jacobson et al., 2006*). 5) Preclinical vaccine development Recombinant vesicular stomatitis virus expressing murine cytomegalovirus gB: As a recombinant vaccine vector, vesicular stomatitis virus (VSV) can induce strong humoral and cellular immunity, particularly at mucosal surfaces. This attribute makes recombinant VSV (rVSV) an attractive candidate for development of a vectored HCMV vaccine (Wilson etal., 2008). Live rVSV vector expressing a murine CMV homolog of the gB protein has been tested in the mouse model (Wilson etal., 2008). This induced neutralizing antibody responses, and resulted in reduced viral titers. Also, splenocytes from immunized mice produced a CD8+ IFN-ÃŽÂ ³ response to gB (Sung and Schleiss, 2010). Recombinant modified vaccinia virus Ankara: The attenuated poxvirus, modified vaccinia virus Ankara (MVA), was established as a safe and potent antigen delivery system. Its genome has undergone six major deletions during serial passage (Sung and Schleiss, 2010), which, in turn, allows the insertion of multiple HCMV genes (Wang et al., 2007). A recombinant MVA vaccine that expresses a soluble, secreted form of HCMV gB, based on the AD169 strain sequence has been constructed (Wang et al., 2004). High levels of gB-specific neutralizing antibodies were elicited in vaccinated mice (Sung and Schleiss, 2010). A trivalent MVA expressing gB, pp65 and IE1 has been developed (Wang et al., 2006) with ability to induce humoral and cellular immunity to gB (Wang et al., 2006). Recombinant MVAs have also been generated expressing both full-length pp65 and exon 4 of IE1 with induction of robust primary cell-mediated immunity and stimulation of vigorous expansion of memory Tcell responses to both antigens (Wang et al., 2007). Another recombinant MVA expressing pp65 and a fusion protein of HCMV IE1 exon 4 and IE2 exon 5 was constructed to maximize the representation of IE-specific immunity (Wang et al., 2008). Replication-deficient adenovirus-vectored polyepitope vaccine: Systemic and mucosal immunity to MCMV could be induced by intranasal immunization using a replication deficient adenoviral vector expressing murine CMV glycoprotein H in a murine model (Shanley and Wu, 2005). Modified adenoviral vector Ad5F35, Ad5F35-AD-1, has been generated, expressing the immunodominant antigenic domain-1 epitope of HCMV gB based on the sequence from the AD169 strain (Zhao et al., 2009). Since the AD-1 epitope is well conserved between different strains of HCMV (Britt et al., 2005), expression of the AD-1 epitope from AD5F35 elicits neutralizing antibody responses to diverse clinical isolates (Zhao et al., 2009). Another replication deficient adenoviral-vectored vaccine, Ad-gBCMVpoly (Zhong et al., 2008) which encodes 46 HCMV T-cell epitopes from multiple antigens covalently linked to the extracellular domain of HCMV gB antigen (Zhong et al., 2008). This chimeric vaccine elicited neutralizing antibody responses and virus-specific CD4+ and CD8+ T-cell responses (Zhong and Khanna, 2009). Recombinant live CMV vaccine by bacterial artificial chromosome mutagenesis: An ideal live-attenuated HCMV vaccine should grow to high titers in cell culture for easy production, should be severely attenuated in vivo, even in immunocompromised hosts, and should elicit a strong immune response sufficient to protect against HCMV-associated disease (Mohr et al., 2008). An approach to the generation of such a vaccine is the targeted deletion of CMV genes modulating the host immune response (Cicin-Sain et al., 2007). This approach has been facilitated by the advances in mutagenesis of cloned CMV genomes maintained as bacterial artificial chromosomes in Escherichia coli as well as the rapidly expanding knowledge about the role of viral genes in immunopathogenesis and immune evasion (Dunn et al., 2003).

Communism :: Communism Essays

The lure of easy women almost made me a spy and traitor to my country "Sex Lead Me To Communism" from Man to Man Vol. 2, No. 2, 1951 SEX, woman's most resistless weapon through the ages for the domination of the male, has proven one of the most successful and insidious devices in the secret arsenal of the Communists to recruit their unsavory army of spies, saboteurs and disruptionists in the grimly declared war on the United States and all decent mankind. In the relentless effort of the Kremlin to turn the whole world into faceless slaves in order that "The International Soviet shall be the human race," as their official song, The Internationale, declares, there is no honor or conscience, and promiscuity is the rule, not the exception. The only vice the Reds frown, upon is drinking! I know, because I was one of those witless persons who fell into the mantrap set by the modern Delilabs who follow the Party line. They stopped at nothing--absolutely nothing--in order to enlist their fellow Americans into the great conspiracy to subjugate, and later to liquidate, every human being who does not deify the unholy trinity of Marx, Lenin and Stalin. First came Sonia, with her full, cherry lips and her brown, limpid eyes. She was a secretary, a graduate of a woman's college and as unscrupulous a little witch as ever shook her clenched fist at a passing flag of her country or stuck a pin into a patrolman's horse. Next came Margie, a full-bosomed redhead, a new comer from England and an expert dress designer. She had made it her life work to lure men into Communism. Then Mildred, a honey blonde with a Vassar degree. She was the gay and sophisticated type on the surface, but underneath she was deadly serious about her radical beliefs. She had a cozy apartment and seemingly plenty of money. She liked to discuss the social significance of Shakespeare over cocktails and make converts to the "cause." Then Terry, Betty and several others whose names I have forgotten. And lastly, Gladys, a banker's daughter who had run away from home, landed in New York's Greenwich Village and become the most radical of all the Communist girls I met. Talk about free love! This was really something. You tired of one, and there was always another at your beck and call. No Turk could boast a more variegated harem.

Police Essay

Police generally perceive the risk of injury, assault, and even death to be greatest for domestic violence calls. Why do police have these beliefs, and what is the reality of the situation? When responding to calls few inspire more dread among police officers than answering to a domestic call. Police have generally the perception of risk of injury, assault and even death. There is always this perception among police officers when answering domestic calls. Such perception cannot be avoided because domestic calls most often than not have the element of unpredictability. Emotions are high and this can run even higher if coupled with drugs and alcohol. Indeed, responding to a domestic call can be very risky on the part of the police officers. When they respond to such call, they are perceived as the threat instead of being the protector. Traditionally, a police officer would respond to a call and restore peace as soon as possible and then clear from the call. The community policing philosophy dictates that the officer review of the problem, analyze it and try to come up with a solution to the problem. (Denise Papagno). However, this theory is not realistic at all. Upon responding to a domestic call, the police officer enters into a domestic abode as a protector, but in the eyes of the owners of the abode despite his good intentions, he will always be the intruder. Hence, the police officer cannot effectively do his job especially if the victim refuses to leave and instead would insist in not doing anything or even file a complaint for that matter. It is essentially difficult for police officers to face and respond to domestic calls as society now views domestic calls as a serious crime and not merely a private family quarrel. The fact that not all of the police are educated in handling domestic issues or why the victim does not leave her abuser or why the batterer abuses the victim. Because of this, the police fail to understand victims of domestic calls and are less inclined to help them in the next call. In your opinion, what is the most problematic myth associated with policing? Explain your answer. As early as the 18th century, police work or policing is associated in being a night watchman. The job basically entails maintaining order against threats to order itself – wild animals, fire or unruly behaviour. However, at the turn of the century, policing has evolved into something more than being a night watchman. Police not only has to maintain order in their respective communities but they are also tasked to solve and fight crimes. Hence, the notion that police can solve any crime reported to them grew. As crime fighters, this notion has proven to be an additional burden to policing. Even simple and private nuisances such as littering, drunkenness and unruly behaviour are called in and reported to the police. Hence, this takes up on the workload of policing instead of focusing more on the serious crime-solving work. But together with the notion of being crime-fighters came the myth of police brutality. The average citizen sees policemen as having â€Å"zero-tolerance†. For example, in domestic calls, instead of turning in their abuser, victims would opt not to do anything for fear of the police. If they fear their abusers, victims fear the police more. It does not help that there are also police mandates such as that of Rudy Giuliani who implemented a version of Broken Windows policing across New York City. Thus, policemen were tasked to become aggressive in stamping out public disorder such as drunkenness, jaywalking and noisiness. In reality however, policing is not just being brutal and aggressive towards those lawbreakers. The concept of â€Å"community policing† has made advancement in policing. Thus, police officers who are on foot patrol have elevated the common myth about policing because admittedly, an officer who is inside the squad car and safely sealed inside cannot relate with the other people from the neighbourhood.

Lord of the Flies- Basic Legal Notions essays

Lord of the Flies- Basic Legal Notions essays The movie the Lord of the flies reflects many basic legal notions. Many of these concepts are identified and questioned throughout the film. The movie starts out with a plane crash. Due to this many private-school boys are left stranded on a deserted island. The boys are left to fend for themselves with no adult authority figures left with them. Piggy and Ralph are the 1st to meet up with each other after escaping from their shot-down plane. They become friends immediately and stick loyally together throughout the movie. They then meet up with Jack and his Choir, Simon, Sam and Eric, and many other characters join in an assembly. Rules are set down, and Ralph is elected to be chief. Ralph calls another assembly, and reminds everyone that they are completely alone on the island, and there are no adults. They are in a state of anarchy (lawlessness). After allocating jobs, laws were loosely set in place to ensure that a society should lightly emerge. Jack and his crew were busy tracking a pig when they were meant to be monitoring the fire. As a result of their lack of involvement they missed an opportunity to get saved by a plane flying ahead. Ralph presses that keeping the signal fire is much more important than hunting and they have displayed a lack of fairness. After a successful hunting expedition, the boys return with a pig which is shared even among the boys, this represent equality as the portions were fair for everyone. Soon, after many more disagreements between Ralph and jack had emerged, they separated into two tribes, Jack went down the savage path turning his crew savage with him. Covering themselves with war-paint, their values consisted of hunting for food and protecting their base camp. Ralphs tribe was based more on ethics; his tribe contained the younger boys and regarded safety and being saved more then a power hierarchy. After a series of murders including Piggys, Jack ...

The Presidents Role in the U.S. Federal Budget Process

The Presidents Role in the U.S. Federal Budget Process The annual federal budget process begins the first Monday in February of each year and should be concluded by October 1, the start of the new Federal Fiscal Year. In some make that most years, the October 1 date is not met. Here is how the process is supposed to work. The President Submits a Budget Proposal to Congress In the first step of the annual U.S. federal budget process, the President of the United States formulates and submits a budget request for the upcoming fiscal year to Congress. Under the Budget and Accounting Act of 1921, the president is required to submit his or her proposed budget to Congress for each government fiscal year, the 12-month period beginning on October 1 and ending on September 30 of the next calendar year. Current federal budget law requires the president to submit the budget proposal budget between the first Monday in January and the first Monday in February. Typically, the president’s budget is submitted during the first week of February. However, especially in years when the new, incoming president belongs to a different party than the former president, submittal of the budget may be delayed. The president’s budget proposal may also be delayed by pressing government financial difficulties. For example, President Barack Obama did not submit his FY 2014 budget proposal until April 10, 2013, due to ongoing negotiations with Congress over the implementation of the budget sequester and mandatory spending cuts dictated by the Budget Control Act of 2011. In fiscal year 2016, the federal budget called for the expenditure of nearly $4 trillion. So, as you might imagine, deciding exactly how that much taxpayer money is to be spent represents a major part of the president’s job. While the formulation of the president’s annual budget proposal takes several months, the Congressional Budget and Impoundment Control Act of 1974 (the Budget Act) requires that it be presented to Congress on or before the first Monday in February. In formulating the budget request, the president is assisted by the Office of Management and Budget (OMB), a major, independent part of the Executive Office of the President. The president’s budget proposals, as well as the final approved budget, are posted on the OMB website. Based on the input of the federal agencies, the presidents budget proposal projects estimated spending, revenue, and borrowing levels broken down by functional categories for the coming fiscal year to start on October 1.The president’s budget proposal includes volumes of information prepared by the president intended to convince Congress that the president’s spending priorities and amounts are justified. In addition, each federal executive branch agency and independent agency includes its own funding request and supporting information. All of these documents are also posted on the OMB website. The presidents budget proposal includes a suggested level of funding for each Cabinet-level agency and all programs currently administered by them. The presidents budget proposal serves as a starting point for the Congress to consider. Congress is under no obligation to adopt all or any of the Presidents budget and often makes significant changes. However, since the President must ultimately approve all future bills they might pass, Congress is often reluctant to completely ignore the spending priorities of the Presidents budget. House and Senate Budget Committees Report the Budget Resolution The Congressional Budget Act requires passage of an annual Congressional Budget Resolution, a concurrent resolution passed in identical form by both House and Senate, but not requiring the Presidents signature. The Budget Resolution is an important document providing Congress an opportunity to lay out its own spending, revenue, borrowing and economic goals for the coming fiscal year, as well as the next five future fiscal years. In recent years, the Budget Resolution has included suggestions for government program spending reforms leading to the goal of a balanced budget. Both the House and Senate Budget Committees hold hearings on the annual Budget Resolution. The committees seek testimony from presidential administration officials, Members of Congress and expert witnesses. Based on testimony and their deliberations, each committee writes or marks-up its respective version of the Budget Resolution. The Budget Committees are required to present or report their final Budget Resolution for consideration by the full House and Senate by April 1. Next Steps: Congress Prepares its Budget Resolution

Research Paper Assignment Example | Topics and Well Written Essays - 1250 words

Research Paper - Assignment Example The government gave a report, and estimated the number of the gas leakage victims was five hundred thousand people (Ferrara, 2012). Three thousand eight hundred of these people had partial injuries, while three thousand nine hundred were permanently injured. There are numerous reasons advanced by scholars as to the cause of the Bhopal disaster. However, most agree that the immediate cause of the disaster was poor management and negligence by the managerial team of Union Carbide India Limited. This paper analyzes the causes of the Bhopal Disaster, in relation to poor managerial and communication skills (Ferrara, 2012). With concrete examples, and well supported facts, this paper looks at how the disaster occurred, and the failure of the management to initiate preventive measures that will protect its workers, from risks emanating from the plant (Rosencranz, 1988). This paper takes a stand that poor management and communication policies at Union Carbide India resulted to the occurrence of the disaster. Failure in communication, before, during and after the Bhopal disaster led to the death of thousands people. In 1984, the Indians comprised of the entire employees at the company. It is the Indians who controlled the machines, and they were involved at virtually all the operations of the company (Dhara et al, 2002). This is because the company implemented its agreement with the Indian government of promoting self-sustenance. On this note, language was a barrier in effectively conducting the affairs of the company. This people lived with their families near the company, and they were the first victims of the gas leakage. Despite this language barrier, the company printed the operating manuals for their equipment’s in English. This was an aspect of mismanagement and negligence; this is because the local community was not proficient in English, and had no idea on know how to react to the warnings contained in the manuals,

Effectiveness of Mentoring in Organizations Essay

Effectiveness of Mentoring in Organizations - Essay Example The performance of employees is dependent upon the degree of alignment achieved with the strategic business objectives which is measured effectively by innovative means in the modern organization. However, it is not easy for the younger talents to understand the detailed aspects of such alignments on their own whereas they bring to table some excellent competencies as an outcome of their respective educational backgrounds. Such talents require effective mentoring to help them align with the organizational goals such that they can gradually build a career in the system by effectively delivering results in line with the strategic objectives of the organization. The mentors need to be those senior experienced colleagues who have been star performers in the system by virtue of their recognized contribution to the goals of the organization. The mentor-protg relationships in organizations, however, are complex and require a lot of organizational focus (as a part of larger strategies) to en sure successful results in building talents for the future. There are numerous benefits to organizations that establish formal mentoring programs within the system and carefully managing the emotional dynamics between the mentors and the protgs by avoiding conflicts of interests. Some organizations practice such strategies for every level in the organization after recognising the need for such programs and clearly setting out their objectives. Such activities are normally carried out through Human Resources department having dedicated functional areas for training & development within the organization. Such a department possesses a senior management representative for effective communication to the top and also possesses their own quality goals in achieving people development in the system. The Human Resources function views the entire organization as a social system and tries to establish models of existing human dynamics prevailing within the organization. These dynamics are then mapped with the growth path that has been established within the organization as per the business requirements in order to build aspirations and healthy competition among people. The employees are then provided guidance and help through senior & experienced employees through well designed mentoring programmes such that every

The Essay Example | Topics and Well Written Essays - 4000 words

The - Essay Example However, with the regime change successfully happening in Egypt, it is acting as a catalyst for even more pro-democracy and anti-monarchy movements throughout the Middle-East. Bahrain is one of those countries, which has been witnessing anti-monarchy movement or uprising. When the protests in Egypt gained momentum, support for them came wide across the Middle-East. In Bahrain also, a group of individuals gathered in front of the Egyptian embassy to support the Egyptian protestors. In addition, they also wanted more political freedom, equal representation to all people and actualization of democratic practices. Political freedom in the sense, with the Bahraini King nominating the Prime Minister and sizable numbers of parliament representatives, demonstrators wanted more ‘elected’ representatives. Importantly, they wanted equal rights and facilities for all the people, particularly for the majority Shias, as minority Sunni section of the population is playing a dominant ro le and garnering all the powers and benefits. So, this paper will discuss how the uprising in Bahrain started or happened due to certain social factors, as well as geographical, historical and political factors, and how it effected and is still impacting the Middle Eastern region. Geographical and Historical factors Bahrain is an archipelago in the Persian Gulf, with Saudi Arabia, Qatar and Iran surrounding it. It consists of 33 islands and the largest is the Bahrain Island, which constitutes the majority of the Bahrain’s population. The capital Manama is situated in the northern tip of the island and is the seat of political power, and thus the hotspot of the uprising. Bahrain got evolved geographically as well as historically due to certain events that happened in the 19th and 20th century and those events can also be viewed as an indirect contributing factor to the current uprising. Bahrain existed as a fragmented geographical territory in the 19th century, with various ki ngdoms. Britain at that time was at the height of its imperialistic tendencies, and it tried to wrest some of the territories. Being a Shia Muslims majority country, Bahrain sought the aid of Iran to counter British imperialism. Although, Iran shielded Bahrain initially, it could not protect it continuously and so British took over Bahrain. Britain brought Bahrain under its protectorate in the Persian Gulf, playing a dominant role in every aspect of Bahrain’s functioning, from appointing its rulers to changing its demographics. Changing of demographics in the sense, British brought in people from Iran, Huwala, as well as India into Bahrain for commercial purposes. â€Å"Mixed with the indigenous population ( in Manama) are numerous strangers and settlers, some of whom have been established here for many generations back, attracted from other lands by the profits of either commerce or the pearl fishery† (Gardner 2010, p. 36). Although, these people brought in with them various business skills, and developed Bahrain from a tribal society to a modern one, it started changing the demographics, as there was heightened concentration of people from outside of Bahrain. The same charge of bringing people from other countries and how is affecting the local population is being levelled by the protestors of the current upris

Psychology Research Essay Example for Free

Psychology Research Essay What are the main points of both articles? Anxiety Disorder: Panic Attacks. It can happen anytime, anywhere. Unexpectedly, out of the blue, your heart starts to speed up, your face goes red and you go through shortness of breath. One feels dizzy, nauseated and out of control. A number of people even think like theyre dying. Such may be a person experiencing a panic attack — a sudden occurrence of powerful fear that induces grave physical reactions in the body (Campbell, 2002). Several people, believing theyre having a heart attack, go to the emergency room. Others try to disregard the signs and symptoms, not comprehending that theyre experiencing a panic attack. A lot of women than men are disturbed by panic attacks. One-month prevalence estimates for women are 0.7% versus 0.3% for men (i.e., women are more likely to be affected than men by a 2- to 3-fold factor) (Daniels et al, 2006). And some people are experiencing frequent panic attacks, a condition known as panic disorder. Panic Anxiety Disorder starts most often when people are 20-30 years old. It activates less often in teenagers or persons in their forties. It is unusual for the disorder to emerge in the elderly for the initial time. Though panic attacks were once set aside as nerves or stress, theyre now documented as a potentially immobilizing, but treatable health condition. An assortment of approaches, together with medications, therapy and relaxation methods, can help one manage or prevent panic attacks (Wilson, 2005). Article #2: Children with Anxiety Disorder. Life is not easy for children these days. The fact that many adults suffer from mental illness of different kinds is enough to make people realize that children are even more susceptible to the same disease. It’s only good that a lot of breakthroughs are made especially in the area and study of mental illness. This study is even more significant in the light of the film Ordinary People, which has captured audiences worldwide decades ago. Precisely because the characters depict the â€Å"ordinariness† of each person and how representative they are to the masses, made this film a hit during its time. Ordinary People presents how malfunctioning individuals are as they go through each of their lives with the burden of mental illness. The role of the mother in the film shows someone who has personality disorder, specifically dissociative disorder, while the spouse and father as having generalized anxiety disorder; and their son Conrad, as suffering with PTSD or Posttraumatic Stress Disorder. Mental health experts describe the illness through its signs and symptoms. It is typified by children experiencing severe fear – a powerful worry or the uneasiness that run or last for periods of time ((http://www.nimh.nih.gov). When children sufferers are not treated, symptoms like these will present themselves through poor performance in academics, inability to relate with other children, low self-esteem, the use and abuse of illegal drugs including alcohol, and even anxiety disorder in adulthood (http://www.nimh.nih.gov).   Mental health experts describe the illness through its signs and symptoms. It is typified by children experiencing severe fear – a powerful worry or the uneasiness that run or last for periods of time ((http://www.nimh.nih.gov). When children sufferers are not treated, symptoms like these will present themselves through poor performance in academics, inability to relate with other children, low self-esteem, the use and abuse of illegal drugs including alcohol, and even anxiety disorder in adulthood (http://www.nimh.nih.gov). This mental health problem usually runs in families. Oftentimes, the disorder appears alongside other physical and mental health problems; substance abuse, like alcohol, usually comes along (http://www.nimh.nih.gov) with it. Anxiety disorder manifests in different forms: 1.) Generalized Anxiety Disorder or GAD, 2.) Phobias, 3.) Separation Anxiety Disorder, 4.) Panic Disorder, 5.)OCD or Obsessive Compulsive Disorder, and 6.) Posttraumatic Stress Disorder (PTSD) (Source: US Dept of Health, 1999 in http://mentalhealth.samhsa.gov/). 2. What are the similarities and/or differences? Similarities come in the form of the disorders’ signs and symptoms which include physical distress with Rapid heart rate , sweating, trembling , shortness of breath and hyperventilation, chills, hot flashes, nausea, abdominal cramping, chest pain, headache, dizziness, faintness, tightness in your throat, trouble swallowing, a sense of impending death . Other health problems — such as an impending heart attack, an overactive thyroid gland (hyperthyroidism) or drug withdrawal — can cause similar signs and symptoms. Individuals who experience panic attacks often are affected by other mental health conditions, including depression, fear of public places (agoraphobia) and social phobia (http://www.emedicine.com/med/topic1725.htm). What experiences have you had that relate to the topic or what have you learned in prior courses? Researchers are not certain what bring about panic attacks. Heredity, stress and certain biochemical factors may play a part. A person’s chance of having panic attacks increases if she/he has a close family member who has had them. Numerous researchers think the bodys natural fight-or-flight reaction to danger is involved. For instance, if a snarling dog came after a child, his/her body would react automatically. The heart rate and breathing would race as the body readies itself for a life-threatening situation. A lot of the same responses take place in a panic attack. No noticeable stressor is nearby, but something trips the bodys alarm system. Persons having Panic attacks are no more likely than the average American to have suffered from emotional problems at the time the disorder begins (Wilson, 2005). How does your view point relate to the topics?) Definitely, I agree from personal observation that these panic attacks and /or anxiety disorder though present only in the mind, â€Å"effectively’ renders a person â€Å"paralyzed† no matter his intelligence, status and persuasion in life. Imagine this picture: after months or years of incessant panic attacks and the constrained lifestyle produced by the distinctive avoidance behavior, the sufferer may develop into a demoralized and psychologically or physically depressed person. In addition, some sufferers resort to alcohol in an effort to self medicate or to reduce the sign and symptoms of the disorder. This significantly causes difficulties to the individuals life and ability to seek suitable treatment. Unfortunately, one out of every five untreated sufferers attempts to end his or her life, not at all comprehending that there was chance for changes and treatment (Wilson, 2005). As in the film as mentioned, it is interesting to note that despite such a troubled family, Conrad emerged to be someone who has conquered the odds in his life. The loss of a brother and its effects on him, being around with parents who exhibit a variety of symptoms that they themselves are also suffering, these things could have been too much for him or for anybody for that matter. PTSD and other anxiety disorder sub-categories are very interesting types of mental health problems because of their prevalence – and in spite of that, still, many people most of the times, ignore the fact that they might be sufferers. Just as in the case of Conrad, ordinary people – the most common among the populace – are actually beset with extremely upsetting events and are left to themselves to cope as each day comes.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   To end, this study from researchers Craske et al shows the hope for sufferers: â€Å"With practice and encouragement or persuasion from the therapist the patient learns to reinterpret internal sensations from signals of loss of control and panic to cues that are intrinsically harmless and can be controlled with certain skills. The intentional creation of these signs by the client, coupled with success in coping with them, reduces their unpredictability and changes their meaning for the client† (Craske, Maidenbberg, Bystritsky, 1995). Reference: For Children with Anxiety Disorder: Campbell, Nancy M. Panic disorder. Mankato, MN: LifeMatters, [2002].  616.85 DYI VT Craske, MG; MAidenberg, E Bystritsky A (1995). Brief Cogntive-behavioral versus nondirective therapy for panic disorder. Journal of Behavior Therapy Experimental Psychiatry ; 26, 113-120. Giacobello, John. Everything you need to know about anxiety and panic attacks. New York: Rosen, [2000]. Daniels C., Yerkes S., Bureau of Naval Personnel emedicine.2006. Wilson, Reid D., Don’t Panic: Taking Control of Anxiety Attacks The Revised Edition Of: Harper Perennial ISBN# 0-06-095160-5. For panic Attacks Article: Davison, Gerald C., Neale, John 2001, Abnormal Psychology, Eighth Ed., John Wiley Sons, Inc. New York. National Institute for Mental Health (NIMH) accessed in the Internet February 20, 2007 http://www.nimh.nih.gov. Surgeon general accessed in the internet February 20, 2007 http://www.surgeongeneral.gov/library/mentalhealth/chapter3/sec6.html.

Purification of Immunoglobulin G by Ion-Exchange

Purification of Immunoglobulin G by Ion-Exchange Purification of Immunoglobulin G by Ion-Exchange Chromatography and Immunoelectropheresis William McTavish Joseph Zappa Introduction Immunoglobins or, Antibodies, are soluble proteins secreted from host differentiated plasma cells that target and eliminate specific antigens to protect the host from disease (Jakoby, 1971). There are five isotypes of immunoglobulin: IgM, IgD, IgA , IgE and IgG, with IgG being the most prominent antibody found in blood circulation of the host. The purification of specific antibodies has led to the development of techniques such as western blotting; where desired proteins can be targeted by monoclonal antibodies engineered for a specific affinity for that protein( Burnette, 1981). The basis of immunoglobulin purification can begin with a technique of â€Å"salting out†, used vastly for precipitating organic molecules and is the first step in protein purification (Tsutomu and Timasheff, 1984). Immunoglobins are small soluble proteins that can be found within serum that is removed from a blood sample taken from the host. Hydrophillic immunoglobins contain amino acids that are polar or possess an ionic charge. Counter ions in the serum of the host are attracted to these polar and ionic charges making the proteins soluble in the solvent. By destabilizing the intermolecular forces between the immunoglobins and the serum solvent there can be an induced precipitation of these proteins. Ammonium sulfate is a highly used compound in salting out procedures, for when ammonium sulfate dissociates, the large sulfate ions form hydrogen bonds between the polar molecules found in the serum (Tsutomu and Timasheff, 1984). The quenching effect of sulfate removes hydrogen bonds and intermolecular forces away from the protein molecules, forcing them to form bonds between one another. This forced intermolecular bonding between proteins causes an accumulation of aggregated proteins and eventually, at the right concentration of salt, precipitation out of solution (Tsutomu and Timasheff, 1984). Although the precipitation of immunoglobulin from host serum with Ammonium sulfate is an efficient procedure for isolating globin, it does not allow for the accurate determination of a specific isotype of immunoglobulin. Ion exchange chromatography is a prominent technique used to acquire a single desired protein, including a specific isotype of immunoglobins. All molecules, including immunoglobulin that have ionizable groups have a net surface charge that is highly dependent on the environmental pH in which that molecule is in. The pH of an environment can dictate the amount of charge present on a molecule, whether it is more positive or more negative, as well as neutral (Grodzki, and Berenstein, 2010). The neutral point, where all positive charges cancel out the negatives is expressed as the pI of the molecule (Grodzki, and Berenstein, 2010). Since all proteins vary in their pI they will express specific charges at any specific pH. This characteristic of immunoglobulin is utilized in Ion exchange chromatography to isolate specific isotypes even if they vary only slightly in charge. IgG, as well as other isotypes of Ig, have a pI occurring near neutral pH so Anion exchange resins are often used for this type of chromatography. Anion Exchangers utilize resin that contains positively charged functional groups that act as counter ions towards protein being eluted through the column (Determann et al. 1969). With the resin set at a specific pH, the proteins that are most positive will exit the column first due to the repulsion of charges between the positive protein and positive resin. The next proteins to elute will be the neutral ones followed by the negatively charged proteins. Proteins are removed in this manner by constantly adding more of the buffer the column is immersed in. By adding more buffer there is an increased competition for associating with the resins charges, which in turn dissociates protein from the resin and further elutes them through the column (Determann et al. 1969). Not only does the charge of the beads matter but also the flow and porosity of the resin, alternations of these can allow for either a more broaden column exchange or a far more refined one. Diethyl aminoethyl (DEAE)-cellul ose is a commonly used resin for anion exchanging due to its higher porosity and positive functional groups that allows for better flow properties of the column. Increased flow rate allows for separation of more bulky and crude proteins, such as crude immunoglobulin, and aids in a higher resolution of separated proteins (Determann et al. 1969). Once several fractions of the column elution is collected there is many ways to identify which fraction is most likely containing the desired protein of isolation including determining the optical density of the fractions with a spectrophotometer. The OD of Immunoglobulin and other proteins can be determined by selecting a specific wavelength of light and beaming it through the elution fraction and recording the amount of transmitted light via photoreceptors (Edelhoch, 1967). A common wavelength used for identifying immunoglobulin is 280nm, this wavelength is absorbed by the amino acid tryptophan in proteins. Absorption of this wavelength in protiens makes it a proportional reduction of transmitted light based on the concentration of protein present in the column fraction (Edelhoch, 1967). The higher the reduction in transmitted light, the higher the OD reading for a fraction. A fraction of elute from Ion exchange chromatography may contain the desired Immunoglobulin G, but to further prove this, a technique called Immunoelectrophoresis (IEP) can be used to confirm the purity of Immunoglobulin fraction. Immunoelectrophoresis is a two-part technique that combines the use of electrophoresis and zone of equivalence of immune complexes to determine a positive result. Electrophoresis is another basic technique used in separating proteins based on size and charge to obtain separate sections of protein in agar gel or other resins such as polyacrylamide in SDS-PAGE techniques.(1) Proteins separate into a gradient of smallest more positive charged towards the cathode to smallest most negative charged towards the anode, with the larger, less charged proteins in the middle gradient. (Serwer and Wright, 2012). After protein separation has occurred in the welled samples, there is addition of antibody specific for certain protein that may be isolated out of the samples used in the experiment. If proteins are present that are the target of affinity for the added antibodies there will be association of antibody:antigen complexes. These complexes will form in the agar gel and at the proper gradient of both antibody and antigen concentrations there will be precipitation of these complexes out of the solution (Slater, 1975). This correct gradient is called the zone of equivalence and is frequently used in determining the presence of desired protein molecules, including immunoglobulin (Slater, 1975). Several other techniques are used in isolating proteins, an extremely prominent technique is the use of Antibodies themselves in Immunofluorescence (IF). Antibodies are engineered to contain a specific affinity towards a desired molecule, protein or even a whole cell. IF can work in either two ways: the first involves a single antibody engineered towards a desired antigen containing a flurochrome itself and emits fluorescent light to be detected. The second contains a secondary antibody that has affinity for the primary antibody binding to an antigen, this secondary antibody is the one that contains the fluochrome for detection (Johnson, 2006). In either of these techniques there is the advantage of staining samples of proteins or cells and identifying not just a single antigen but several with several different antibodies. This technique is extremely useful for identifying proteins in cell structures as well as identifying the presence of proteins in biological systems. Methods and Materials All methods used in this experiment can be located in the Immunology Laboratory Manual cited in references. There were two major alternations to the Immunoelectrophoresis experiment; There was a time of 1.5 to 2 hours allowed for electrophoresis of the agar slides instead of 1 to 1.5 hours. There was also an expansion of time from 24 hours to 48 hours allowed for the IEP slide to rest in a cold room before soaking in 1% NaCl solution. Results A high concentration of IgG was isolated in the third elution fraction from DEAE-cellulose Ion exchange chromatography. Optical density of six Ion exchange chromatography elution fractions were taken with a spectrophotometer to determine protein concentration at a wavelength of 280nm (Fig 1). The highest optical density was observed in the third elution fraction (Fig 1). This illustrates that the largest concentration of protein at a similar charge was eluted at the third fraction of the Ion exchange experiment. Figure 1. Third fraction of DEAE-celluose elution scored the highest optical density. All fractions were tested with spectrophotometry and optical density measurements were taken at a wavelength of 280nm (Fig 1). Results are shown as single values of optical density (OD) and relate to the amount of protein concentration in each fraction. (Fig 1) Immunoelectrophoresis of isolated protein reveals presence of purified IgG in response to Goat anti-rabbit serum Presence of Rabbit Immunoglobin was tested for using Immunoelectrophoresis with Goat anti-rabbit serum. Normal rabbit serum and purified fraction of protein were welled on a 1% agar slide and proteins were separated based on charge via electrophoresis. Anti-rabbit serum was added and results were taken for precipitation of immune complexes 48 hours later (Fig 2). Thin white lines between the wells and trough are precipitated immune complexes and thus show a positive test for rabbit immunglobins (Fig 2). Figure 2. Precipitated immune complexes reveal immunoglobin presence in normal rabbit serum and purified fraction. Proteins were isolated based on charge via electrophoresis to isolate specific proteins. Goat Anti-rabbit serum was added as antibody for rabbit immunoglobin and incubated for 48 hours. Distinction of grey and white bands are positive results regardless Discussion Purified Rabbit Immunoglobin G was isolated from Normal rabbit serum using DEAE-cellulose ion exchange chromatography and Immunelectropheresis with Goat anti-rabbit serum. Once the majority of proteins were salted out of the normal rabbit serum, Ion exchange chromatography was used to separate all proteins from the sample of crude globin. Since immunoglobin proteins are soluble in the blood and are near neutrally charged at philological pH, a large amount of protein was expected to elute roughly half way through the Ion exchange chromatography regardless of using anion or cation exchange columns (Grodzki and Berenstein, 2010). These results occurred for the DEAE-cellulose Ion exchange column used to separate crude rabbit globulin in our experiment. The third elution fraction, of six, contained the highest optical density when evaluated with the spectrophotometer at 280nm. Optical density is related to the concentration of protein in a sample, thus the fraction containing the highest amount of protein was the third fraction which was collected half way through the elution process. Although the method of determining sample concentrations for proteins can vary, these results can be seen in similar protein isolation studies such as Ye et al. article Isolation of lactoperoxidase, lactoferrin, ÃŽ ±-lactalbumin, ÃŽ ²-lactoglobulin B and ÃŽ ²-lactoglobulin A from bovine rennet whey using ion exchange chromatography. The protein isolated is presumed to be the globulin isotype Iummogloublin G, this is due to the nature of circulating antibodies found in the serum of the rabbit. The most prominent antibody isotype circulating in the blood is IgG, which binds to antigens, forming immune complexes as well as aiding in many other immune system mechanisms such as compliment activation, opsonization and etc (Collins and Jackson, 2013). Immunoelectrophoersis with Goat anti-rabbit serum was used next to determine whether or not the isolated protein in the third elution fraction is Immunoglobulin G. The nature of this experiment depends on two key process gel electrophoresis and precipitation of Immune complexes. If electrophoresis is preformed properly there should be a separation of proteins based on charge/size from the samples that were welled on the agar covered slide used in the experiment; creating small zones of protein purity along the slide (Slater, 1975). Since the eluted fraction sample should only contain one kind of protein and is roughly pure, there should only be one zone of protein sample, where the normal rabbit serum, containing an array of different proteins, will electrophoresis out into several different zones of protein. Determining these zones of protein was done by adding Goat anti-rabbit serum and allowing diffusion into the gel to create zones of equivalence between antibody and antigen, thu s precipitating the complex to be seen visibly (Serwer and Wright, 2012). For a positive result on the purity of the fraction sample only a single precipitation line formed at the zone of equivalence would be seen. The results for the purity of the fraction sample was conclusive with the above expectations, only a single faint precipitated line was seen on the gel; therefore re-enforcing that there is only a single protein isolated from the Ion exchange elution phase. The single protein isolated is promptly IgG due to it’s response to the anti-Rabbit serum containing anti-rabbit globulin. Immunelectrophoresis was used in this experiment to confirm the presence of IgG in the eluted fraction sample taken from DEAE-cellulose ion exchange chromatography. The reason this method was used was due it’s simplicity in determining specific immune complexes and thus re-ensuring purity. It is relatively quick in determining the presence of antigen, in this case the immunoglobin G of rabbit, and gives results ready to be read visually, lacking the need for software or other means of identification. The draw back of this technique is that it takes some practical skill in preparation and is only useful in identifying the purity of one sample at a time. Techniques such as western blotting would be more efficient for studies that desire more than a single purity such as Yang et al’s article Correlation between the overexpression of epidermal growth factor receptor and mesenchymal markers in endometrial carcinoma. An alternation to this experiment could be made in the chroma Purification of Immunoglobins is an extremely useful procedure. Being able to isolate specific classes of Immunoglobulin aids in research of host immune deficiencies such as the research done by Tamura et al in their article Tumor-Produced Secreted Form of Binding of Immunoglobulin Protein Elicits Antigen-Specific Tumor Immunity as well as many other fields of host immunity and clinical research. Successful purification and crystallization of Immunoglobulin has also allowed for insight on how host immune systems respond to infection and the biological processes that take place in these responses. References Jakoby, W.B. 1971. Cystallization as a purification technique, Enzyme Purification and Related Techniques, Methods in Enzymology. 22: 246-252 Determann, H. Meyer, N. Wieland, T. 1969. Ion exchanger from pearl-shaped cellulose gel. Nature 223: 499-500 Edelhoch, H. 1967. Spectrospoic determination of tryptophan and tyrosine in proteins Burnette, N.W. 1981. â€Å"Western Blotting†: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochem 112: 1935-203 Tsutomu A. and Timasheff, S.N. 1984. Mechanism of protein salting in and salting out by divalent cation salts: balance between hydration and salt binding Biochemistry(23)25:5912-5926 321 -Grodzki, A.C. Berenstein, E. (2010) Antibody Purification: Ion-Exchange Chromatography Methods in Molecular Biology 588: 27-32 Slater, L. 1975. IgG, IgA and IgM by formylated rocket immunoelectrophoresis. Ann Clin Biochem 12 (1) : 19-22, 24 Yang, W.N.Ai, Z.H. Wang, Xu, J.Y.L. Teng, Y.C. 2014.Correlation between the overexpression of epidermal growth factor receptor and mesenchymal makers in endometrial carcinoma. J Gynecol Oncol. 25:36-42. 47 Collins, A.M. Jackson, K.J.L. 2013. A temporal model of human IgE and IgG antibody function. Front Immunol 4: 225 Ye, X. Yoshida, S. Ng, T.B. 2000. Isolation of lactoperoxidase, lactoferrin, ÃŽ ±-lactalbumin, ÃŽ ²-lactoglobulin B and ÃŽ ²-lactoglobulin A from bovine rennet whey using ion exchange chromatography The international journal of Biochemistry Cell biology 32 (11-12): 1143-1150 22 Nydegger, U.E. Lambert, P.H. Gerber, H. Miescher, P.A. 1974. Circulating Immune Complexes in the Serum in Systemic Lupus Erythematosus and in Carriers of Hepatitis B Antigen QUANTITATION BY BINDING TO RADIOLABELED Clq Circulating immune complexes in the serum in Systemic Lupus Erythematosus and in carriers of Hepatitis Antigen B Quantitation by binding to Radiolabelled Clq. J Clin Invest.  54(2): 297–309. Serwer, P. Wright, E.T. 2012. Agarose Gel Electrophoresis Reveals Structural Fluidity of Phage T3 DNA Packaging Intermediate. Electrophoresis 33 (2): 352-365 101-Johnson, I.D. 2006. Practical considerations in the selection and application of fluorescent probes. In: Handbook of biological confocal microscopy, 3rd ed. (J.B. Pawley.ed), Plenum Press. new York. p.362-3. Circulating Immune Complexes in the Serum in Systemic Lupus Erythematosus and in Carriers of Hepatitis B Antigen QUANTITATION BY BINDING TO RADIOLABELED Clq